NGG1 Interacting Factor 3 Like Protein 1 (NIF3L1)

The mouse and human cDNAs encode deduced proteins of 376 and 377 amino acids, respectively, that share 84% sequence identity. Both proteins show strong homology to proteins of lower species, indicating a high degree of conservation from bacteria to mammals.Northern blot analysis showed ubiquitous expression of mouse Nif3l1, with a major transcript size of 1.85 kb. RT-PCR analysis demonstrated ubiquitous expression of Nif3l1 throughout mouse embryonic development. Expression analysis on cell lines revealed strong overexpression in a spermatogonia-derived cell line and a teratocarcinoma cell line. A human NIF3L1/GFP fusion protein showed localization of the protein in the cytoplasm.