Prostate And Breast Cancer Overexpressed Protein 1 (PBOV1)

Sequence analysis predicted that the 135-amino acid protein contains a transmembrane domain, 3 potential phosphorylation sites, and 1 potential myristoylation site. Northern blot analysis revealed expression of a 2.0-kb transcript in colon, prostate, small intestine, testis, and spleen, with lower expression in thymus, ovary, and peripheral blood leukocytes. A 2.4-kb transcript was also detected in prostate and spleen. RT-PCR analysis detected upregulated expression of UROC28 in prostate, breast, and bladder cancer, but not in lung and colon cancer, compared with normal tissue. In situ hybridization analysis showed preferential expression of UROC28 in prostate and breast glandular or ductal epithelial cells and elevated, heterogeneous expression of UROC28 in prostate and breast tumors. Immunohistochemical analysis detected primarily cytoplasmic as well as nuclear expression.