Reprimo (RPRM)

RPRM is induced following x-irradiation in a p53-dependent manner and is a downstream mediator of p53 action. Overexpression of RPRM in HeLa cells by transfection resulted in G2 arrest of the cell cycle by inhibiting both CDC2 activity and nuclear translocation of the CDC2-cyclin B1 (CCNB1) complex.Using a differential display screening approach on mouse embryonal fibroblasts to identify genes that are induced by p53 following x-irradiation, Ohki et al. (2000) identified one, which they termed Reprimo, that is involved in cell cycle regulation. Ohki et al. (2000) cloned mouse and human Reprimo cDNAs, which encode deduced 109-amino acid proteins sharing 98% sequence identity. The Reprimo protein is N-glycosylated and localizes to the cytoplasm.