Sjogren Syndrome Nuclear Autoantigen 1 (SSNA1)

The predicted 119-amino acid protein has a calculated molecular mass of 13.6 kD and has an N-terminal dimeric coiled-coil domain and a C-terminal basic domain.SSNA1 shares 96.6% amino acid identity with its mouse homolog. Northern blot analysis of adult human tissues detected strong expression in testes, with moderate to low expression in spleen, thymus, prostate, ovary, small intestine, colon, and peripheral blood leukocytes. Immunofluorescence microscopy of HeLa and mouse 3T3 cells transfected with SSNA1 showed numerous punctate structures scattered throughout the nucleus.Anti-DIP13 antibody specifically recognized NA14, staining basal bodies and flagella of human sperm cells and the centrosome of HeLa cells.