TGFB1 Induced Anti-Apoptotic Factor 1 (TIAF1)

The deduced 2,035-amino acid protein shares 94% homology with KIAA0216, but it contains an additional 458 N-terminal amino acids. human TIAF1 may also encode a longer protein. Mouse Tiaf1 contains an N-terminal KE-rich region, followed by a PDZ domain, a head region with an ATP-binding site, a neck region with an IQ motif, and a coiled-coil C-terminal domain. Northern blot analysis detected Tiaf1 in all tissues examined. A 10.5-kb transcript was detected in heart and skeletal muscle, and a 7.0-kb transcript was detected in hematopoietic tissues and cells. Western blot analysis detected Tiaf1 at an apparent molecular mass of 230 kD. Immunostaining localized Tiaf1 in a filamentous network slightly concentrated in the perinuclear region of mouse stromal cells.