Testis Expressed Protein 35 (TEX35)

The full cDNA length of mouse Tsc24 was 899 bp, with a 624 bp open reading frame encoding a 207 amino acid protein with a predicted molecular weight of 23.997 kDa.The results of semi-quantitative RT-PCR showed that the expression of mouse Tsc24 can only be detected after the mouse was 35 d old and the expressing level increased gradually from day 35 to 6 months. Of the eight tissues examined in mice, and of the 12 tissues examined in human, Tsc24 was exclusively expressed in the testis, but in none of the other studied tissues. The result of subcellular localization of GFP-Tsc24 fusion protein in Cos-7 cells supports that Tsc24 protein is expressed in nuclear.