Testis Specific Y Encoded Like Protein 3 (TSPYL3)

TSPYL3, Belongs to the nucleosome assembly protein (NAP) family. TSPYL2, TSPYL3, and TSPYL6 were transcriptionally silenced but induced by 5-azadC alone or 5-azadC plus TSA treatment. TSPYL1 and TSPYL4 expression was readily detected at levels comparable with normal brain. TSPYL2, TSPYL3, and to a lesser extent TSPYL6, however, were down-regulated in most of the tumor samples.  TSPYL genes were highly expressed in both whole adult and fetal brain. The TSPYL genes were variably expressed in other tissues, with expression of TSPYL4 and TSPYL5 being more ubiquitous and TSPYL1, TSPYL2, TSPYL3, and TSPYL6 being more restricted. Collectively, these results reveal that the TSPYL family of genes is highly expressed in normal brain, and that TSPYL2, TSPYL3, and TSPYL6 may also be subject to frequent epigenetic silencing in brain tumors.