Unc-93 Homolog A (UNC93A)

UNC93A gene contains 8 exons and spans 24.5 kb. Alternative splicing results in 2 transcripts, one of which lacks exon 4. The full-length UNC93A cDNA encodes a deduced 457-amino acid protein with a predicted molecular mass of 50 kD. The protein contains a leader peptide followed by 7 transmembrane domains. It shares 30% sequence identity with the C. elegans protein.Northern blot analysis revealed a major transcript of approximately 2.1 kb in testis, small intestine, and colon; a smaller transcript of 1.1 kb was also detected in spleen, prostate, ovary, small intestine, and colon. No expression was detected in 8 ovarian cancer cell lines. Transient expression of a GFP-UNC93A fusion protein in 293T cells showed localization to the membrane.