Gametogenetin Binding Protein 2 (GGNBP2)

The deduced 741-amino acid mouse Dif3 protein has a calculated molecular mass of 84 kD and contains a C2H2 zinc finger domain, 2 basic regions, and 3 repeated acidic regions. Northern and Western analysis detected mouse Dif3 in testis only, where it was expressed during spermatogenesis, most strongly in large pachytene spermatocytes, as determined by immunohistochemical analysis. The deduced 288-amino acid human protein has a calculated molecular mass of 32.7 kD. Northern blot analysis of polyA+ mRNA detected a 3.4-kb transcript with strong expression in human heart, pancreas, skeletal muscle, and kidney, with lower expression in liver, lung, placenta, and brain. Northern blot analysis of mouse tissues detected strong expression in testis and liver with lower expression in all other tissues examined.