AHNAK Nucleoprotein 2 (AHNAK2)

AHNAK2 gene contains at least 7 exons. The first 6 exons are relatively small, while exon 7 is almost 18 kb. The deduced 600-kD protein contains an N-terminal PDZ domain, followed by 24 repeats of about 165 amino acids each and a C-terminal segment of about 100 kD. Each repeat likely folds into anti-parallel beta strands to generate tandemly connected propeller-like units. AHNAK2 also has 10 potential SH3-binding sites. Immunolocalization showed Ahnak2 localized to Z-band regions of mouse heart, where it colocalized with alpha-actinin. Subcellular fractionation of mouse myocardium found Ahnak2 localized with Ahnak in nuclei and myofibrillar aggregates that included Z-band material and in a vesicular fraction that included a T-tubule marker.