ATP Binding Cassette Transporter C10 (ABCC10)

Members of the ABC family of membrane proteins, such as ABCC10, are involved in energy-dependent transport of a wide variety of substrates across membranes. The 1,492-amino acid ABCC10 protein has a calculated molecular mass of 162 kD. It contains 2 nucleotide-binding folds, 17 predicted transmembrane segments arranged in 3 transmembrane domains, and 3 predicted N-glycosylation sites. Northern blot analysis was unable to detected ABCC10 expression, but RT-PCR detected ABCC10 expression in all 10 tissues examined. In vitro-translated ABCC10 had an apparent molecular mass of 158 kD by SDS-PAGE.Basal MRP7 promoter activity relied upon a proximal segment of the 5-prime flanking region bearing an E2F site acting cooperatively with 2 closely positioned SP1 sites.