Abelson Helper Integration Site 1 (AHI1)

AHI1 contains a coiled-coil domain in its N-terminal 140 amino acids. These amino acids and this domain are entirely missing in the predicted proteins of both mouse and rat, but are present in the predicted proteins of nonhuman primates and other mammals.Full-length human AHI1 contains at least 1,187 amino acids. Both the rodent and human proteins have 7 WD40 repeats and an SH3 domain, but human AHI1 contains an N-terminal coiled-coil domain not found in the rodent proteins. One of the human AHI1 splice variants encodes a protein lacking the SH3 domain. RT-PCR analysis of Jurkat human T cells identified splice variants exhibiting exon skipping in the 5-prime UTR. Northern blot analysis of mouse tissues detected several Ahi1 splice variants, with highest expression in brain and testis and very low expression in liver.