Activating Transcription Factor 7 Interacting Protein 2 (ATF7IP2)

Conserved domains 1 and 2 of MCAF1 and MCAF2 share 27% and 56% amino acid identity, respectively. Database analysis revealed that domain 2 shows highest conservation between species. Western blot analysis of several human cell lines detected variable ATF71IP2 expression at an apparent molecular mass of 100 kD. Fluorescence-tagged MCAF1 and MCAF2 colocalized with MBD1 at nuclear foci.Immunoprecipitation analysis of K562 human erythroleukemia cells revealed that MCAF1 showed higher affinity for SETDB1 than SP1, whereas MCAF2 showed higher affinity for SP1 than SETDB1. Both MCAF proteins were also detected in the same immunoprecipitates. Both MCAF1 and MCAF2 increased transcription of a reporter gene activated by SP1, and the TRD of MBD1 moderately repressed transcription in the presence of either MCAF1 or MCAF2