Adaptor Related Protein Complex 2 Alpha 2 (AP2a2)

By randomly sequencing cDNA clones from size-fractionated human brain cDNA libraries, Nagase et al. (1998) identified AP2A2, which they designated KIAA0899. The deduced 929-amino acid protein shares more than 97% sequence identity with rat alpha-adaptin. By RT-PCR analysis followed by ELISA for quantitation, they detected ubiquitous, low-to-moderate expression of KIAA0899, with highest levels in spinal cord and fetal and adult brain and lowest levels in spleen.Using the N terminus of huntingtin (HTT) in a yeast 2-hybrid screen of a testis cDNA library, Faber et al. (1998) identified AP2A2, which they designated huntingtin yeast partner J (HYPJ). The predicted protein shares significant sequence identity with mouse alpha-adaptin C.