AlkB, Alkylation Repair Homolog 6 (ALKBH6)

By database analysis to identify sequences similar to ABH genes, followed by PCR of a testis cDNA library, Tsujikawa et al. (2007) cloned ALKBH6, which they called ABH6. Other than a short N-terminal segment, the deduced 169-amino acid protein consists entirely of an AlkB homology domain that is predicted to function as a 2-oxoglutarate- and Fe(II)-dependent oxygenase domain. Quantitative real-time PCR detected variable ABH6 expression in all 16 tissues examined, with highest expression in testis and pancreas. Fluorescence-tagged ABH6 was expressed in both the cytoplasm and nucleus of transfected HeLa cells.Hartz (2010) mapped the ALKBH6 gene to chromosome 19q13.12 based on an alignment of the ALKBH6 sequence with the genomic sequence (GRCh37).