Ankyrin Repeat And Sterile Alpha Motif Domain Containing Protein 1A (ANKS1A)

ANKS1 expression in all tissues examined, with highest expression in heart, skeletal muscle, and pancreas. KG-1 and HeLa cell lines also showed high ANKS1 expression.The deduced 1,134-amino acid protein has a calculated molecular mass of about 123 kD. It has 6 N-terminal tandem ankyrin repeats, followed by 2 sterile alpha motif (SAM) domains and a C-terminal phosphotyrosine-binding (PTB) domain. Northern blot analysis detected a 6.0-kb transcript in nearly all tissues and cell lines tested. Western blot analysis of mouse tissues and human and rodent cell lines detected Odin expressed at an apparent molecular mass of 130 kD. Immunofluorescence and Western blot analysis found Odin was expressed as a cytoplasmic protein.