Ankyrin Repeat Domain Protein 1 (ANKRD1)

Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy.The deduced 319-amino acid protein has a calculated molecular mass of 36 kD. ANKRD1 contains a nuclear localization signal, 4 tandem ankyrin-like repeats, multiple phosphorylation sites, and a PEST-rich region, which is typically found in rapidly degraded proteins. The 3-prime UTR of ANKRD1 has numerous AT-rich mRNA decay elements. Northern blot analysis of multiple human cell lines detected ANKRD1 expression in microvascular and large vessel endothelial cell lines. Immunofluorescence of transfected COS cells and immunoprecipitation of nuclear and cytoplasmic extracts of COS cells showed that ANKRD1 was abundantly expressed in nucleus.