Ankyrin Repeat Domain Protein 54 (ANKRD54)

The deduced 299-amino acid protein has an N-terminal ATP-binding P loop, followed by a nuclear localization signal, 4 ankyrin repeats, and a C-terminal nuclear export signal. It has several potential serine phosphorylation sites. Mouse and human LIAR share 92% amino acid identity. Northern blot analysis detected variable Liar expression in all mouse tissues examined, with highest expression in testis. Variable mRNA and protein expression was also detected in all erythropoietic cell lines examined. In situ hybridization of mouse embryos at embryonic day 10 revealed Liar expression in brachial arches, maxillary process, fore and hind limb buds, and gastrointestinal tract.Domain analysis revealed that the SH3 domain of Lyn and the ankyrin repeat domain of Liar were required for the interaction.