Antagonist Of Mitotic Exit Network Protein 1 (AMN1)

AMN1 required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN). The Dbf2 protein kinase functions as part of MEN, which controls the inactivation of the Cdc28-Clb2 kinase in late mitosis. The MEN includes the Tem1 GTP binding protein; the kinases Cdc15 and Cdc5; Mob1, a protein of unknown function; and the phosphatase Cdc14. Tem1 acts at the top of the pathway, upstream of Cdc15, which in turn functions upstream of Mob1 and Dbf2. The Cdc5 Polo-like kinase impinges at least twice on the MEN since it negatively regulates the network, probably upstream of Tem1, and is also required again for Dbf2 kinase activation.In metaphase-arrested cells, the MEN inhibitor Bub2 restrains both Dbf2 kinase activity and actin ring formation.