Apolipoprotein L Domain Containing Protein 1 (APOLD1)

APOLD1 is an endothelial cell early response protein that may play a role in regulation of endothelial cell signaling and vascular function. The deduced human APOLD1 protein contains 348 amino acids and shares 87% identity with the 246-amino acid rodent Apold1 proteins. APOLD1 contains a predicted N-terminal leucine zipper motif, 2 hydrophobic regions, a C-terminal coiled-coil domain, and several predicted phosphorylation sites. In situ analysis detected high levels of Apold1 mRNA in the rat embryonic heart and decreased levels in adult heart with expression restricted to capillaries and the inner surfaces of arteries, and veins. Dual fluorescent cell labeling with an endothelial cell marker demonstrated endothelial cell localization of Apold1 in vessels of all rat tissues sampled.