Armadillo Repeat Containing Protein 10 (ARMC10)

ARMC10 contains an armadillo repeat, an N-terminal cell attachment sequence, an N-terminal transmembrane domain, and 3 PKC and 6 casein kinase II phosphorylation sites. Northern blot analysis detected a major 2.6-kb transcript in all tissues tested with high expression in placenta, liver, kidney, heart, and brain. RT-PCR analysis identified 4 splice variants, resulting from alternative splicing of exons 2 and 5, which they designated SVHA, SVHB, SVHC, and SVHD. Confocal microscopy and immunofluorescence studies localized all splice variants to the endoplasmic reticulum membrane, and the N-terminal transmembrane domain was required for ER subcellular localization. Quantitative RT-PCR showed that only SVHB, which lacks exon 2, was upregulated in hepatocellular carcinoma samples.