Arrestin Domain Containing Protein 2 (ARRDC2)

Arrdc2 with a GC content of 65%, was amplified from gut cDNA by PCR with PfuTurbo polymerase in the presence of 1.1 M betaine and 4 mM Mg2 and subcloned into pcDNA3.1/V5/His. Human Arrdc4 as “down-regulated in hepatocellular carcinoma 1 (DRH1)”, Rat Arrdc2 as “induced by lysergic acid diethylamide-1 (ILAD-1)” , and most recently, human Arrdc3 as “thioredoxin-binding-protein-2-like inducible membrane protein (TLIMP)” . The most distantly related protein, Arrdc5, is notable for its sperm-specific expression. The functions of these proteins and the significance of the arrestin domains remain unknown. Alignment of the three proteins (Arrdc2– 4) with the most identity to Txnip demonstrated a single conserved cysteine.Thioredoxin-GST failed to detect an interaction with either Arrdc2 or Arrdc4 under conditions that bound Txnip.