Basic Leucine Zipper And W2 Domain Containing Protein 1 (BZW1)

BZW1 was highly expressed in MEC tissues and cells, determined by quantitative real time polymerase chain reaction. Higher levels of BZW1 proteins were detected in MEC tissues, particularly in high-grade MEC, by immunohistochemistry. Furthermore, down-regulation of BZW1 expression by infection with BZW1-specific RNAi-expressing lentivirus inhibited Mc3 cell proliferation and colony formation in vitro. down-regulation of BZW1 expression arrested Mc3 cell cycling at the G(0)/G(1) phase and mitigated Mc3 cell migration and invasiveness in vitro, determined by wound healing and transwell invasion assays. Importantly, down-regulation of BZW1 expression significantly reduced the tumorigenicity of Mc3 cells in vivo, evidenced by the slow progression of small tumors induced by BZW1-specific RNAi expressing Mc3 cells.