BolA Like Protein 2 (BOLA2)

By searching databases for sequences similar to E. coli BolA, followed by RT-PCR of total brain RNA, Zhou et al. (2008) cloned human BOLA1, BOLA2, and BOLA3. The deduced 152-amino acid BOLA2 protein has a BolA domain containing a helix-turn-helix motif near its C terminus. RT-PCR analysis detected variable BOLA2 expression in all normal human tissues examined. BOLA2 was secreted into the culture medium of transfected COS-7 cells.Kasai et al. (2004) determined the solution structure of mouse Bola2. The overall topology is a class II KH fold, except that it lacks the well-conserved GxxG loop. The residues conserved in BolA-like proteins appeared to assemble on 1 side of the protein.