C-Type Lectin Domain Family 9, Member A (CLEC9A)

CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells. The predicted type II transmembrane CTLR contains 241 amino acids and has a calculated molecular mass of 30 kD. It has a C-type lectin domain (CTLD), followed by a stalk region, the transmembrane domain, and a cytoplasmic tail with an immunoreceptor tyrosine-based activation motif (ITAM). The CTLD contains 6 characteristic cysteines, but it lacks residues involved in calcium ion coordination and carbohydrate binding. The stalk region has 2 cysteines and an N-linked glycosylation site. Flow cytometric, Western blot, and deglycosylation analyses showed surface expression of a glycosylated CLEC9A dimer of 40 and 45 kD under reducing conditions.