CCR4 NOT Transcription Complex Subunit 1 (CNOT1)

The human NOT1 ESTs contained partial coding sequences. In their studies, the authors used a partial NOT1 cDNA, which they referred to as NOT1C, that was obtained by 5-prime extending a NOT1 EST. The NOT1C cDNA encodes an N-terminally truncated protein of approximately 1,300 amino acids that corresponds to two-thirds of the predicted NOT1 protein. Northern blot analysis detected an approximately 8-kb major NOT1 transcript in all human tissues examined, namely brain, heart, lung, liver, kidney, small intestine, colon, spleen, thymus, peripheral blood leukocytes, skeletal muscle, and placenta. Like the expression of human NOT2, NOT3, and CALIF, NOT1 expression was high in brain, kidney, and placenta and very low in skeletal muscle and colon.