Centrosomal Protein 135kDa (CEP135)

The transcript contains repetitive elements at both the 5-prime and 3-prime UTRs, and the deduced protein contains 846 amino acids. RT-PCR detected variable CEP135 expression in all tissues examined, with highest expression in kidney, testis, and ovary, and lowest expression in brain and spleen. CEP135, PLK4, SAS6, CPAP (CENPJ), TUBG1, and CP110 were required at different stages of procentriole formation and were associated with different centriolar structures. SAS6 associated only transiently with nascent procentrioles, whereas CEP135 and CPAP formed a core structure within the proximal lumen of both parental and nascent centrioles. The predicted molecular mass was 135 kD. Imaging confirmed that fluorescence- and epitope-tagged CEP135 localized to centrosomes in a human osteoblastoma cell line.