Chromosome 19 Open Reading Frame 25 (C19orf25)

C19orf25, Belongs to the UPF0449 family. Mass spectrometric analysis of eluates of the tandem affinity purification of ZW10LAPtag and Zwint-1LAPtag from mitotically arrested cells showed that the two proteins resided in distinct kinetochore complexes. ZW10, however, resided in a complex with known interacting partners Rod and Zwilch. In addition, Rad50-interactor-1, a protein proposed to bind ZW10 in interphase, was present. So too was a novel, previously hypothetical protein FLJ36666/C19ORF25. Neither Rad50-interactor-1 nor C19ORF25 bound kinetochores when fused to EYFP (unpublished data), however, supporting the likelihood that they are part of an interphase ZW10-containing protein structure.