Chromosome 9 Open Reading Frame 64 (C9orf64)

By searching for genes in a region of chromosome 9q commonly deleted in acute myeloid leukemia (AML), followed by PCR of CD34-positive bone marrow cells, Sweetser et al. (2005) cloned C9ORF64. The deduced protein contains 289 amino acids. Sweetser et al. (2005) determined that the C9ORF64 gene contains 4 exons. By genomic sequence analysis, Sweetser et al. (2005) mapped the C9ORF64 gene to chromosome 9q21.32Ten ovarian cancer cell lines were analyzed by methylation-specific PCR, and seven (GPR150, LOC222171, PRTFDC1, LOC339210, ITGA8, C9orf64 and HOXD11) of the 33 CGIs were methylated in one or more of the cell lines. Demethylation of methylated cell lines with 5-aza-2′-deoxycytidine restored expression of two genes (PRTFDC1 and C9orf64).