Cytoskeleton Associated Protein 5 (CKAP5)

By sequencing clones obtained from a size-fractionated immature myeloid cell line cDNA library, Nagase et al. (1995) cloned CKAP5, which they designated KIAA0097. The deduced protein contains 2,032 amino acids. Northern blot analysis detected highest expression in skeletal muscle and lower expression in all other tissues and cell lines examined, except peripheral blood leukocytes.Using TACC1 as bait in a yeast 2-hybrid screen of a mammary epithelial cDNA library, Lauffart et al. (2002) cloned CKAP5, which they called CHTOG. CHTOG contains a microtubule-binding domain in its N-terminal half and a KXGS motif for binding tubulin dimers in its C-terminal half.