DDB1 And CUL4 Associated Factor 6 (DCAF6)

NRIP also enhanced AR- and GCCR-mediated transcriptional activity in a reporter gene assay in the presence of AR or GCCR ligands. Downregulation of endogenous NRIP by siRNA reduced the growth rate of a human prostate carcinoma cell line in the absence of ligand. The deduced 860-amino acid protein has a calculated molecular mass of 96 kD. It contains 7 WD40 repeats, 5 of which are N-terminal and 2 of which are C-terminal following a nuclear localization signal. Northern blot analysis detected highest expression in skeletal muscle and testis, and weaker expression in heart, prostate, and adrenal gland. NRIP was also detected in all cancer cell lines examined. Fluorescence-tagged NRIP was expressed in the nuclei of transfected human embryonic kidney cells.