DENN/MADD Domain Containing Protein 5B (DENND5B)

DENND5B Belongs to the RAB6IP1 family.Contains 1 dDENN domain.Contains 1 DENN domain.Contains 1 PLAT domain.Contains 2 RUN domains.Contains 1 uDENN domain. Rab6 regulates vesicular cargo at the level of Golgi via interactions with numerous and unrelated effector proteins. The crystal structure of Rab6a(GTP) in complex with a 378-residue internal fragment of the effector Rab6IP1 (Rab6 Interacting Protein 1) has been solved at 3.2Å resolution. This region of Rab6IP1 encompasses an all a -helical RUN domain followed in tandem by a PLAT domain that adopts a b -sandwich fold. The structure reveals that the first and last a -helices of the RUN domain mediate binding to switch I, switch II and the interswitch region of Rab6. The structure represents the largest Rab-effector complex determined to date.