DNA Cross Link Repair 1A Protein (DCLRE1A)

DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1A is one of several evolutionarily conserved genes involved in repair of interstrand cross-links.The deduced 1,040-amino acid protein shares 33.3% identity over 192 amino acids with the S. cerevisiae DNA repair protein Snm1. Northern blot analysis detected expression in all tissues and cell lines examined except peripheral blood leukocytes.DCLRE1A contains a nuclear localization signal and a zinc finger motif in its N-terminal half and 8 motifs shared with SNM1-like proteins of various species in its C-terminal half. Fluorescence-tagged SNM1 localized to nuclei of transfected human fibroblasts and Chinese hamster ovary cells, but it was excluded from nucleoli.