DNA Damage Regulated Autophagy Modulator 2 (DRAM2)

The deduced 266-amino acid DRAM2 protein shares 45% identity with DRAM1. Northern blot analysis detected variable expression of DRAM2 in most tissues examined, with highest expression in placenta and heart and little to no expression in brain and thymus. Immunohistochemical analysis showed that DRAM2 colocalized with a lysosomal marker.The deduced 266-amino acid protein has 6 putative transmembrane domains and a unique 21-amino acid C-terminal region not found in DRAM1. Western blot analysis detected endogenous DRAM2 at an apparent molecular mass of 35 kD in HEK293 cells. Immunohistochemical analysis showed that DRAM1 and DRAM2 colocalized in lysosomes.