Deltex Homolog 2 (DTX2)

The deduced 740-amino acid protein shares a high degree of similarity with human DTX1. RT-PCR ELISA detected DTX3 expression in all tissues and specific brain regions examined, with highest expression in lung, liver, spleen, testis, and thalamus. The deduced full-length DTX2A protein contains 622 amino acids and has an N-terminal domain predicted to associate with Notch ankyrin repeats, followed by a proline-rich region with potential Src homology-3 (SH3) domain-binding sites and a C-terminal domain that includes a classic RING-H2 finger motif. Compared with DTX2A, DTX2B lacks part of the proline-rich regionrecombinant DTX2 functioned as a ubiquitin E3 ligase. It showed highest ubiquitination activity in conjunction with the E2 enzyme UBCH5A (UBE2D1).