Discoidin, CUB And LCCL Domain Containing Protein 2 (DCBLD2)

ESDN transcript was upregulated by PDGF-BB in a dose-dependent manner in primary cultures of human coronary artery smooth muscle cells. In vivo, rat carotid arteries showed upregulation of Esdn transcript and protein in response to balloon-generated injury. Expression of ESDN in 293T cells caused suppression of cell division, as indicated by reduced bromodeoxyuridine uptake.Western blot analysis identified 3 ESDN protein bands of molecular masses 127, 106, and 93 kD, suggesting that ESDN is N-glycosylated, since all are larger than the calculated molecular mass of 89 kD. Northern blot analysis detected major and minor ESDN transcripts of 6.4 and 3 kb, respectively, with high expression in smooth muscle cells and lower expression in endothelial cells.