Endoplasmic Reticulum Lipid Raft Associated Protein 1 (ERLIN1)

ERLIN1 has a calculated molecular mass of 38.9 kD. It has an N-terminal mitochondrial targeting sequence, followed by a transmembrane region and sites for phosphorylation, N-myristoylation, N-glycosylation, and glycosaminoglycan attachment. Northern blot analysis detected a 3.8-kb transcript in heart, placenta, liver, kidney, pancreas, prostate, testis, ovary, small intestine, and colon. RT-PCR showed variable expression in all cell lines examined. Western blot analysis revealed a 39-kD protein in antigen-activated dendritic cells, but not in unactivated dendritic cells.Immunohistochemical analysis of endogenous and fluorescence-tagged proteins revealed that ERLIN1 and ERLIN2 localized specifically to the endoplasmic reticulum and nuclear envelope.