Family With Sequence Similarity 208, Member A (FAM208A)

Clone se89-1 cDNA showed a 98% similarity to RAP140 in a 3444-bp overlap and a gap of 60 bp, which is located within the ORF resulting in a 20-aa gap. RT-PCR was performed by using various primers: first a combination of primers detected both RAP140 and se89-1 and revealed three bands in testis cDNA and two bands in various other cDNAs. By using this primer together with the forward primer againstRAP140, which also detects se89-1, only one band was amplified. By using Northern blotting, the presence of se89-1-specific RNA was confirmed within RNA derived from brain, kidney, colon, and testis and the CTCL line SeAx (data not shown).