Fer1 Like Protein 5 (FER1L5)

A novel member of the ferlin family, Fer1L5, because of its high homology to dysferlin and myoferlin. Fer1l5 protein is abundantly expressed in nascent myotubes containing only 2–4 nuclei. Fer1l5 protein binds directly to the endocytic recycling proteins, EHD1 and EHD2, and that this interaction is mediated by the second C2 domain in fer1l5. Loss of EHD1 and/or EHD2 inhibits myoblast fusion, and EHD2 is required for normal translocation of fer1L5 to the plasma membrane. Abolishing the nucleotide binding ability of the EHD2 ATPase domain recapitulates the fer1L5 translocation defect. The characterization of fer1L5 and its interaction with EHD1 and EHD2 underscores the complex requirement of ferlin proteins and mediators of endocytic recycling for membrane trafficking events.