Fibronectin Type III And SPRY Domain Containing 1 Like Protein (FSD1L)

The deduced 496-amino acid protein contains a coiled-coil region, followed by a fibronectin type-3 (FN3) domain and a C-terminal B30.2 box, all of which are believed to be protein-protein interaction domains. MIR1 also has a 20-amino acid region between the FN3 and B30.2 domains that has multiple consensus sequences for proline-directed cyclin-dependent serine/threonine kinases. Northern blot analysis of several human tissues detected MIR1 expression only in brain. RNA dot blot analysis detected MIR1 in all areas of adult brain tested, but little expression was found in fetal brain. MIR1 was also expressed at lower levels in fetal and adult thymus, pituitary, and testis, and weakly in several other tissues. Western blot analysis detected endogenous MIR1 at an apparent molecular mass of 65 kD in a human osteosarcoma cell line and in rat and cow brain cytosol.