Functional Spliceosome Associated Protein 33 (fSAP33)

By sequencing clones obtained from a size-fractionated brain cDNA library, Hirosawa et al. (1999) cloned ISY1, which they designated KIAA1160. The deduced protein contains 351 amino acids. RT-PCR ELISA detected expression in all adult and fetal tissues examined, with highest levels in liver, lung, and ovary. Within specific adult brain regions, highest expression was in amygdala and cerebellum.Using a yeast model, Villa and Guthrie (2005) presented evidence that ISY1 regulates the fidelity of pre-mRNA splicing.By radiation hybrid analysis, Hirosawa et al. (1999) mapped the ISY1 gene to chromosome 3.