G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3)

Using a cDNA probe against a mouse/human somatic cell hybrid panel, Sparkes et al. (1987) mapped the alpha inhibiting polypeptide-3 of G protein to chromosome 1. Alpha-3 cDNA codes for a protein of 340 amino acids , of which the sequence is closely related to but distinct from that of alpha-2. Wilkie et al. (1992) assigned the gene to 1p13. They assigned the corresponding gene to mouse chromosome 3 by study of restriction fragment length variation in an interspecific backcross. Baron et al. (1994) demonstrated that the Gnai3 gene in the hamster is less than 60 kb from the Ampd2 gene with which it is coamplified in coformycin-resistant cells. The hamster Gnai3 gene did not contain sequences corresponding to the combined U6 snRNA and E protein pseudogene, previously identified within intron 7 of the human gene.