GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2)

GABARAPL2 contains 117 amino acids and has a calculated molecular mass of 13.7 kD. Like GABARAP, it contains a basic N-terminal tubulin binding domain and a GABA-A receptor binding domain. GABARAPL2 shares 57% identity with GABARAP and 100% amino acid sequence identity with bovine brain GATE16, rat Gabarapl2, and mouse Gabarapl2. Northern blot analysis revealed ubiquitous expression of a 1.35-kb transcript with high levels in heart, brain, testis, prostate, ovary, spleen, and skeletal muscle, and low levels in lung, thymus, and small intestine. GABARAPL2 had activity as a soluble transport factor, it interacted with N-ethylmaleimide-sensitive factor (NSF) and stimulated its ATPase activity, and interacted with the Golgi v-SNARE (GOS28) in an NSF-dependent manner.