GTF2I Repeat Domain Containing Protein 2B (GTF2IRD2B)

The deduced human GTF2IRD2B protein contains 949 amino acids and has a calculated molecular mass of 107 kD. The N-terminal half of GTF2IRD2B contains a leucine zipper motif, followed by 2 helix-loop-helix motifs (I repeats) that share homology with the TFII-I family of transcription factors. The C-terminal half of GTF2IRD2B contains a CHARLIE8 transposable element-like sequence, including 3 transposase-related domains that may be functional, and a BED zinc finger DNA-binding motif. GTF2IRD2B also has 4 N-glycosylation sites and multiple putative phosphorylation sites. The 949-amino acid GTF2IRD2A protein has 6 C-terminal amino acid changes relative to GTF2IRD2B. Northern blot analysis detected a 3.5-kb GTF2IRD2B transcript in all tissues tested, and RT-PCR analysis confirmed ubiquitous expression.