Glutamate Rich WD Repeat Containing Protein 1 (GRWD1)

GRWD1 is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis . The cDNA contains several repetitive elements in its 3-prime end, and the deduced 445-amino acid protein contains a WD domain. The deduced 446-amino acid protein contains a glutamic/aspartic acid-rich domain of 14 consecutive amino acids and 4 WD repeat domains. RNA dot blot analysis showed ubiquitous GRWD1 expression, with highest levels in liver, placenta, a transformed colorectal cell line, and a lung carcinoma cell line. Epitope-tagged GRWD1 was expressed mainly in the nucleus of transduced mouse fibroblasts, but some immunoreactivity was present in cytoplasm. Western blot analysis detected a major GRWD1 band at 62 kD and a minor band at 90 kD.