Golgi Phosphoprotein 3 (GOLPH3)

GOLPH3 encodes a deduced 298-amino acid protein. Immunogold electron microscopy localized rat Golph3 at the periphery of Golgi stacks, on the cis and trans sides, as well as at the lateral edges of stacked cisternae. Scanning confocal microscopy of cultured rat hippocampal neurons and a rat fibroblast cell line confirmed the Golgi localization and more diffuse cytosolic labeling.Upregulation of MIDAS in HeLa cells caused an increase in mitochondria number, redistributed mitochondria to the perinuclear region, and prevented cell growth. MIDAS had no effect on mitochondria membrane potential, cytoskeletal structure, or mitochondrial DNA, RNA or protein, but the amount of the mitochondria-specific phospholipid cardiolipin was increased almost 2-fold following MIDAS overexpression.