Hook Homolog 2 (HOOK2)

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment. The deduced 728-amino acid protein contains an N-terminal domain, a central coiled-coil domain, and a C-terminal domain. HOOK2 shares 30% identity with Drosophila Hook, with highest conservation in the N-terminal domain. Endogenous HOOK2 detected in HEK293 cells had an apparent molecular mass of about 83 kD. Endogenous HOOK2 localized to discrete punctate subcellular structures that were often observed in linear tracks that colocalized with microtubule markers.