Kelch Repeat And BTB Domain Containing Protein 10 (KBTBD10)

By characterizing cDNA clones showing high abundance in an adult skeletal muscle cDNA library, Garrison et al. (1985) identified a partial sarcosin cDNA, which they designated clone P2a. Using this clone as probe, Taylor et al. (1998) identified 2 related sarcosin transcripts in a second adult skeletal muscle cDNA library. The longer transcript contained a 3-prime extension due to use of a second polyadenylation signal. Both transcripts encode a deduced 435-amino acid protein with a calculated molecular mass of about 48.5 kD. Northern blot analysis detected transcripts at 2.3 kb and 2.7 kb in heart and skeletal muscle and not in other tissues. Minor expression was also found in smooth muscle from prostate. Densitometry revealed that the longer transcript represented 57% of the expressed mRNA.