Kidney And Brain Protein (KIBRA)

The deduced 1,113-amino acid protein has a calculated molecular mass of about 125 kD. It contains 2 N-terminal WW domains, and a C2-like domain and glutamine-rich domain in its C-terminal half. Northern blot analysis detected a major 4.3-kb transcript predominantly expressed in kidney and brain. Following expression in green monkey kidney cells, Western blot analysis detected human KIBRA and endogenous Kibra at an apparent molecular mass of 125 kD. Fluorescence microscopy localized KIBRA predominantly to the cytoplasm, with perinuclear enrichment.highest expression in liver and kidney, intermediate expression in brain, lung, pancreas, and ovary, weak expression in heart and testis, and no expression in skeletal muscle and spleen.