Kinesin Family, Member 18A (KIF18A)

KIF18A contains a motor domain and conserved ATP- and microtubule-binding sites, and the central region is predicted to have an alpha-helical coiled-coil, followed by a C-terminal cargo-binding domain. Nuclear localization signals, protein-protein interaction motifs such as SH3, WW, AP-1, and FHA domains, and potential sites for phosphorylation and sumoylation were also identified. KIF18A shares 84% and 81% homology with the mouse and rat homologs, respectively. Indirect immunofluorescence microscopy localized endogenous KIF18A to the nucleus, cytoplasm, and plasma membrane ruffles in preosteogenic marrow stromal cells. Punctate cytoplasmic staining of KIF18A was associated with microtubules and partial colocalization with actin.